| Date | Opponents | Type | Res/KO | Venue |
|---|---|---|---|---|
| Sat 22 Aug 1964 | Leyton Orient | League | L 2-5 | Brisbane Road |
| Wed 26 Aug 1964 | BURY | League | W 2-1 | Fratton Park |
| Sat 29 Aug 1964 | CHARLTON ATHLETIC | League | L 2-3 | Fratton Park |
| Tue 01 Sep 1964 | Bury | League | D 1-1 | Gigg Lane |
| Sat 05 Sep 1964 | Manchester City | League | L 0-2 | Maine Road |
| Sat 12 Sep 1964 | SOUTHAMPTON | League | L 0-3 | Fratton Park |
| Tue 15 Sep 1964 | Rotherham United | League | L 0-1 | Millmoor |
| Sat 19 Sep 1964 | SWINDON TOWN | League | W 5-0 | Fratton Park |
| Sat 26 Sep 1964 | Derby County | League | L 0-4 | Baseball Ground |
| Wed 30 Sep 1964 | ROTHERHAM UNITED | League | W 2-0 | Fratton Park |
| Sat 03 Oct 1964 | SWANSEA TOWN | League | W 1-0 | Fratton Park |
| Wed 07 Oct 1964 | NORTHAMPTON TOWN | League | D 3-3 | Fratton Park |
| Sat 10 Oct 1964 | Huddersfield Town | League | L 1-2 | Leeds Road |
| Sat 17 Oct 1964 | COVENTRY CITY | League | L 0-2 | Fratton Park |
| Sat 24 Oct 1964 | Cardiff City | League | L 0-1 | Ninian Park |
| Sat 31 Oct 1964 | PRESTON NORTH END | League | W 1-0 | Fratton Park |
| Sat 07 Nov 1964 | Ipswich Town | League | L 0-7 | Portman Road |
| Sat 14 Nov 1964 | PLYMOUTH ARGYLE | League | L 0-1 | Fratton Park |
| Sat 21 Nov 1964 | Bolton Wanderers | League | L 2-3 | Burnden Park |
| Sat 28 Nov 1964 | MIDDLESBROUGH | League | W 2-1 | Fratton Park |
| Sat 05 Dec 1964 | Newcastle United | League | L 0-3 | St. James Park |
| Sat 12 Dec 1964 | LEYTON ORIENT | League | D 1-1 | Fratton Park |
| Sat 19 Dec 1964 | Charlton Athletic | League | D 3-3 | The Valley |
| Sat 26 Dec 1964 | Crystal Palace | League | L 2-4 | Selhurst Park |
| Mon 28 Dec 1964 | CRYSTAL PALACE | League | D 1-1 | Fratton Park |
| Sat 02 Jan 1965 | MANCHESTER CITY | League | D 1-1 | Fratton Park |
| Sat 16 Jan 1965 | Southampton | League | D 2-2 | The Dell |
| Sat 23 Jan 1965 | Swindon Town | League | D 0-0 | County Ground |
| Sat 06 Feb 1965 | DERBY COUNTY | League | W 3-1 | Fratton Park |
| Sat 13 Feb 1965 | Swansea Town | League | D 0-0 | Vetch Field |
| Sat 20 Feb 1965 | HUDDERSFIELD TOWN | League | W 3-0 | Fratton Park |
| Sat 27 Feb 1965 | Coventry City | League | W 2-1 | Highfield Road |
| Sat 06 Mar 1965 | NEWCASTLE UNITED | League | L 1-2 | Fratton Park |
| Sat 13 Mar 1965 | Preston North End | League | L 1-6 | Deepdale |
| Sat 20 Mar 1965 | IPSWICH TOWN | League | L 0-2 | Fratton Park |
| Sat 27 Mar 1965 | Plymouth Argyle | League | L 1-2 | Home Park |
| Sat 03 Apr 1965 | BOLTON WANDERERS | League | W 3-0 | Fratton Park |
| Fri 09 Apr 1965 | Middlesbrough | League | L 1-4 | Ayresome Park |
| Sat 17 Apr 1965 | CARDIFF CITY | League | W 1-0 | Fratton Park |
| Mon 19 Apr 1965 | NORWICH CITY | League | W 4-0 | Fratton Park |
| Tue 20 Apr 1965 | Norwich City | League | L 1-3 | Carrow Road |
| Sat 24 Apr 1965 | Northampton Town | League | D 1-1 | County Ground |