| Date | Opponents | Type | Res/KO | Venue |
|---|---|---|---|---|
| Sat 21 Aug 1965 | PLYMOUTH ARGYLE | League | W 4-1 | Fratton Park |
| Mon 23 Aug 1965 | Preston North End | League | L 1-4 | Deepdale |
| Sat 28 Aug 1965 | Southampton | League | D 2-2 | The Dell |
| Wed 01 Sep 1965 | PRESTON NORTH END | League | W 4-1 | Fratton Park |
| Sat 04 Sep 1965 | Bolton Wanderers | League | L 0-2 | Burnden Park |
| Wed 08 Sep 1965 | Crystal Palace | League | L 1-4 | Selhurst Park |
| Sat 11 Sep 1965 | IPSWICH TOWN | League | W 1-0 | Fratton Park |
| Wed 15 Sep 1965 | CRYSTAL PALACE | League | D 1-1 | Fratton Park |
| Sat 18 Sep 1965 | Birmingham City | League | W 3-1 | St. Andrews |
| Sat 25 Sep 1965 | LEYTON ORIENT | League | W 4-1 | Fratton Park |
| Sat 09 Oct 1965 | DERBY COUNTY | League | D 1-1 | Fratton Park |
| Sat 16 Oct 1965 | Cardiff City | League | W 2-1 | Ninian Park |
| Sat 23 Oct 1965 | CARLISLE UNITED | League | W 4-1 | Fratton Park |
| Tue 26 Oct 1965 | Middlesbrough | League | L 2-5 | Ayresome Park |
| Sat 30 Oct 1965 | Coventry City | League | L 2-3 | Highfield Road |
| Sat 06 Nov 1965 | BRISTOL CITY | League | L 2-4 | Fratton Park |
| Sat 13 Nov 1965 | Manchester City | League | L 1-3 | Maine Road |
| Sat 20 Nov 1965 | ROTHERHAM UNITED | League | D 1-1 | Fratton Park |
| Sat 27 Nov 1965 | Wolverhampton Wanderers | League | L 2-8 | Molineux |
| Sat 04 Dec 1965 | NORWICH CITY | League | L 0-3 | Fratton Park |
| Sat 11 Dec 1965 | Bury | League | L 0-1 | Gigg Lane |
| Sat 18 Dec 1965 | CARDIFF CITY | League | W 3-1 | Fratton Park |
| Mon 27 Dec 1965 | Charlton Athletic | League | D 2-2 | The Valley |
| Tue 28 Dec 1965 | CHARLTON ATHLETIC | League | W 3-1 | Fratton Park |
| Sat 01 Jan 1966 | Derby County | League | L 1-3 | Baseball Ground |
| Sat 08 Jan 1966 | MANCHESTER CITY | League | D 2-2 | Fratton Park |
| Sat 15 Jan 1966 | Carlisle United | League | L 1-2 | Brunton Park |
| Sat 29 Jan 1966 | Plymouth Argyle | League | L 1-3 | Home Park |
| Sat 05 Feb 1966 | SOUTHAMPTON | League | L 2-5 | Fratton Park |
| Sat 19 Feb 1966 | BOLTON WANDERERS | League | W 1-0 | Fratton Park |
| Sat 26 Feb 1966 | Ipswich Town | League | L 0-1 | Portman Road |
| Sat 12 Mar 1966 | BIRMINGHAM CITY | League | L 0-1 | Fratton Park |
| Sat 19 Mar 1966 | Leyton Orient | League | D 0-0 | Brisbane Road |
| Fri 25 Mar 1966 | MIDDLESBROUGH | League | W 4-1 | Fratton Park |
| Sat 02 Apr 1966 | Bristol City | League | L 0-1 | Ashton Gate |
| Wed 06 Apr 1966 | HUDDERSFIELD TOWN | League | W 2-1 | Fratton Park |
| Sat 09 Apr 1966 | COVENTRY CITY | League | W 2-0 | Fratton Park |
| Mon 11 Apr 1966 | Huddersfield Town | League | L 0-2 | Leeds Road |
| Sat 16 Apr 1966 | Rotherham United | League | D 3-3 | Millmoor |
| Sat 23 Apr 1966 | WOLVERHAMPTON WANDERERS | League | W 2-0 | Fratton Park |
| Sat 30 Apr 1966 | Norwich City | League | W 3-1 | Carrow Road |
| Sat 07 May 1966 | BURY | League | W 4-0 | Fratton Park |
